HPC:Sailfish
From HPC wiki
Sailfish
Sailfish is a Rapid Alignment-free Isoform Quantification from RNA-Seq Reads
Sailfish v0.6.3 is installed on all the PMACS cluster nodes and is made available as an environment module:
[asrini@consign ~]$ bsub -Is bash [asrini@node061 ~]$ module show sailfish-0.6.3 ------------------------------------------------------------------- /usr/share/Modules/modulefiles/sailfish-0.6.3: module-whatis Rapid Alignment-free Isoform Quantification from RNA-Seq Reads prepend-path PATH /opt/software/sailfish/0.6.3/bin prepend-path LD_LIBRARY_PATH /opt/software/sailfish/0.6.3/lib ------------------------------------------------------------------- [asrini@node061 ~]$ module load sailfish-0.6.3 [asrini@node061 ~]$ sailfish -h Allowed Options: -v [ --version ] print version string --no-version-check don't check with the server to see if this is the latest version -h [ --help ] produce help message Sailfish v0.6.3 =============== Please invoke sailfish with one of the following commands {index, quant, sf}. For more inforation on the options for theses particular methods, use the -h flag along with the method name. For example: Sailfish index -h will give you detailed help information about the index command.