HPC:bcl2fastq

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bcl2fastq

A few different versions of bcl2fastq2 are installed across all HPC nodes. The available versions are: bcl2fastq2-v2.15.0.4, bcl2fastq2-v2.16.0.10 and bcl2fastq2-v2.17.1.14.

Usage

Any of bcl2fastq2 can be loaded as a module:

[asrini@node61 ~]$ module avail

--------------------------------------------------------- /usr/share/Modules/modulefiles ---------------------------------------------------------
AllPaths-LG-v49967              EMBOSS-6.6.0                    openmpi-1.5.4-x86_64            rum-2.0.5_06
autoconf-2.69                   eQtlBma-1.2.2                   pcre-8.38                       sailfish-0.6.3
automake-1.15                   eQtlBma-1.3.1                   perl5lib                        samtools-0.1.18
bamtools-2.3.0                  FastQC-0.11.2                   picard-1.96                     samtools-0.1.19
bcl2fastq2-v2.15.0.4            fastx_toolkit-0.0.14            picard-tools-1.141              samtools-1.1
bcl2fastq2-v2.16.0.10           gsl-2.1                         pkg-config-path                 scons-2.3.1-1
bcl2fastq2-v2.17.1.14           homer-v4.6                      plink-1.90b3b                   SOAPdenovo2-r240
blat-v35                        java-sdk-1.6.0                  python-2.7.5                    sratoolkit-2.4.2
boost-1.55.0                    java-sdk-1.7.0                  python-2.7.9                    STAR-2.3.0e
boost-1.57.0                    kClust-1.0                      python-3.4.2                    STAR-2.4.1d
bowtie2-2.1.0                   ld-library-path                 qiime-1.8.0                     STAR-2.5.2a
bwa-0.7.10                      libtool-2.4.6                   R-3.1.1                         STAR-hg19
bzip2-1.0.6                     local_coreutils-8.24            R-3.1.2                         STAR-mm9
CASAVA-v1.8.2                   manpath                         R-3.2.1                         texinfo-6.0
CGAL-4.7                        module-git                      R-3.2.2                         tophat-2.0.11
circos-0.67-5                   module-info                     R-3.3.0                         Trimmomatic-0.32
cufflinks-2.2.0                 modules                         R-3.3.1                         trinity-r20140717
cufflinks-2.2.1                 NAMD-2.9-Linux-x86_64-multicore r-libs-user                     use.own
curl-7.49.0                     ncbi-blast-2.2.0                ruby-1.9.3-p551                 WiggleTools-1.0
devtoolset-2                    ngsutils-0.5.7                  ruby-2.1.5                      xz-5.2.2
dot                             null                            rum-2.0.5_05                    zlib-1.2.8-1

---------------------------------------------------------------- /etc/modulefiles ----------------------------------------------------------------
mpich-x86_64


[asrini@node061 ~]$ module load bcl2fastq2-v2.17.1.14

[asrini@node061 ~]$  bcl2fastq  --version
BCL to FASTQ file converter
bcl2fastq v2.17.1.14
Copyright (c) 2007-2015 Illumina, Inc.

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