HPC:samtools
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samtools
samtools versions 0.1.18 and 0.1.19 are installed on all HPC nodes.
samtools v0.1.18 is the system default :
[asrini@node061 ~]$ which samtools /usr/bin/samtools [asrini@node061 ~]$ samtools Program: samtools (Tools for alignments in the SAM format) Version: 0.1.18 (r982:295) Usage: samtools <command> [options] Command: view SAM<->BAM conversion sort sort alignment file mpileup multi-way pileup depth compute the depth faidx index/extract FASTA tview text alignment viewer index index alignment idxstats BAM index stats (r595 or later) fixmate fix mate information flagstat simple stats calmd recalculate MD/NM tags and '=' bases merge merge sorted alignments rmdup remove PCR duplicates reheader replace BAM header cat concatenate BAMs targetcut cut fosmid regions (for fosmid pool only) phase phase heterozygotes
If you need to use samtools v0.1.19, use the environment module:
[asrini@node061 ~]$ module load samtools-0.1.19 [asrini@node061 ~]$ which samtools /opt/software/samtools/samtools-0.1.19/samtools [asrini@node061 ~]$ samtools Program: samtools (Tools for alignments in the SAM format) Version: 0.1.19-44428cd Usage: samtools <command> [options] Command: view SAM<->BAM conversion sort sort alignment file mpileup multi-way pileup depth compute the depth faidx index/extract FASTA tview text alignment viewer index index alignment idxstats BAM index stats (r595 or later) fixmate fix mate information flagstat simple stats calmd recalculate MD/NM tags and '=' bases merge merge sorted alignments rmdup remove PCR duplicates reheader replace BAM header cat concatenate BAMs bedcov read depth per BED region targetcut cut fosmid regions (for fosmid pool only) phase phase heterozygotes bamshuf shuffle and group alignments by name