Difference between revisions of "HPC:bclconvert"

From HPC wiki
(Created page with "=== BCL Convert === The Illumina BCL Convert is a standalone local software app that converts the Binary Base Call (BCL) files produced by Illumina™ sequencing systems to FA...")
 
Line 7: Line 7:
  
 
----
 
----
<div class="mw-collapsible mw-collapsed">:
+
<div class="mw-collapsible mw-collapsed">
  
 
<pre>
 
<pre>
Line 48: Line 48:
 
</pre>
 
</pre>
 
</div>
 
</div>
 +
 
=== Other Pages ===
 
=== Other Pages ===
 
----
 
----

Revision as of 19:07, 24 August 2021

BCL Convert

The Illumina BCL Convert is a standalone local software app that converts the Binary Base Call (BCL) files produced by Illumina™ sequencing systems to FASTQ files. Additionally, BCL Convert provides adapter handling (through masking and trimming) and UMI trimming and produces metric outputs.

BCL Convert module usage

To check available versions of BCL Convert, run the "module avail" command (click "expand" to see basic module usage):


[asrini@node155 ~]$ module avail

--------------------------------------------------------------------------------- /usr/share/Modules/modulefiles ----------------------------------------------------------------------------------
AllPaths-LG-v49967              bzip2/1.0.6                     gcc/8.3.1                       NAMD-2.9-Linux-x86_64-multicore R/3.1.1                         samtools-1.1
autoconf/2.69                   CASAVA/v1.8.2                   gsl-2.1                         ngsutils-0.5.7                  R/3.1.2                         scons/2.3.1
automake/1.15                   cellranger/5.0.1                hdf5/1.8.21                     null                            R/3.2.1                         SOAPdenovo2-r240
bamtools/2.3.0                  circos/0.67-5                   homer-v4.6                      pandoc/2.0.6                    R/3.2.2                         STAR/2.3.0e
bcftools/1.11                   cmake/3.21.1                    htslib/1.11                     pandoc/2.3                      R/3.3.0                         STAR/2.4.1d
bcl2fastq2/v2.15.0.4            cufflinks-2.2.0                 java/openjdk-1.6.0              parallel/20201122               R/3.3.1                         STAR/2.5.2a
bcl2fastq2/v2.16.0.10           cufflinks-2.2.1                 java/openjdk-1.7.0              pcre-8.38                       R/3.4.2                         STAR/2.7.1a
bcl2fastq2/v2.17.1.14           curl-7.49.0                     java/openjdk-1.8.0              perl5lib                        R/3.4.3                         texinfo/6.0
bcl2fastq2/v2.19.0.316          dot                             julia/1.0.5                     picard/1.96                     R/3.5.1                         texlive-20170214
bcl2fastq2/v2.20.0.422          EMBOSS-6.6.0                    julia/1.4.2                     picard-tools-1.141              R/3.6.3                         Trimmomatic-0.32
bcl-convert/3.8.2               eQtlBma-1.2.2                   ld-library-path                 pkg-config-path                 R/4.0.2                         trinity-r20140717
blat-v35                        eQtlBma-1.3.1                   libtool/2.4.6                   plink-1.90b3b                   rclone/1.47.0                   use.own
boost/1.55.0                    FastQC-0.11.2                   local_coreutils-8.24            python/2.7.9                    RUM/2.0.5_06                    wget/1.20.3
boost/1.57.0                    fastx_toolkit-0.0.14            manpath                         python/3.6.3                    sailfish/0.6.3                  WiggleTools/1.0
bowtie2/2.1.0                   gcc/5.3.1                       module-git                      python/3.9.1                    samtools/1.11                   zlib/1.2.8
bowtie2/2.3.4.1                 gcc/6.3.1                       module-info                     qiime/1.8.0                     samtools-0.1.18
bwa-0.7.10                      gcc/7.3.1                       modules                         QTLtools/1.3.1-11               samtools-0.1.19

---------------------------------------------------------------------------------------- /etc/modulefiles -----------------------------------------------------------------------------------------
mpi/mpich-3.0-x86_64 mpi/mpich-3.2-x86_64 mpi/mpich-x86_64

To load/use one of the available BCL Convert modules run the "module load" command (click "expand" to see basic module usage):



:

Other Pages